Fig. 8.

PPARγ overexpression impaired osteoblastogenesis and the expression of osteoblast-specific gene expression in vitro. a-e Expression of the osteoblast-related genes Runx2, OCN, ALP, COL1a, and OSX in osteoblasts following the transfection with pcDNA-PPARγ or the empty vector. f ALP expression in osteoblasts and mineralized extracellular matrix was detected using Alizarin Red S staining treated following the transfection with pcDNA-PPARγ or the empty vector for 7 days and 21 days. g The number of ALP-positive cells following the transfection with pcDNA-PPARγ or the empty vector for 7 days. h Osteoblasts were transfected with pcDNA-PPARγ or the empty vector. Cell lysates were analyzed using western blotting. The expression of Runx2, β-catenin, and PPARγ was evaluated. All experiments were performed at least three times. *P < 0.05 and **P < 0.01 compared with the control group