Table 1.

Primers used in this study

Primer	Sequence (5′ → 3′)	Note
Po-gpdF	GGTACCTTTATTGGCGGT	Promoter cloning
Po-gpdR	CCAGGTCAGTGAAATTTCC
pal1_gF	ATGACAATCCTATCCGCAGAC	gDNA fragment cloning
pal1_gR	TTAGAATAAATCAACGATGATA
pal1_cF	ATGACAATCCTATCCGCAGAC	cDNA fragment cloning
pal1_cR	TTAGAATAAATCAACGATGATA
pal2_gF	ATGACTATTCTCTCAGGGA	gDNA fragment cloning
pal2_gR	CTACGCGAACATCGCTA
pal2_cF	ATGACTATTCTCTCAGGGA	cDNA fragment cloning
pal2_cR	CTACGCGAACATCGCTA
pal1-qF	CTCCTTCACAATCGCATCTA	qPCR
pal1-qR	CTTCAGCCGCCTATGTTG
pal2-qF	CAACTGCTGCGTATGTCA
pal2-qR	GATGTAGAGGTATGAGGAGATT
β-actin-F	GCGATGAACAATAGCAGGG	Endogenous control
β-actin-R	GCTGGTATCCACGAGACAAC
pal1-OE-F	ttacaggtcaaagttATGACAATCCTATCCGCAGAC	Construction of OE plasmids
pal1-OE-R	aattctagagggcccTTAGAATAAATCAACGATGATA
pal2-OE-F	ttacaggtcaaagttATGACTATTCTCTCAGGGA
pal2-OE-R	aattctagagggcccCTACGCGAACATCGCTA
pal1-RNAi-F1	actgacctggGATTTGCAACCGTTGTCTTACG	Construction of RNAi plasmids
pal1-RNAi-R1	gttggagtgcaactccaCTAAAATCAGATGAGGTTGTAAGCG
pal1-RNAi-F2	tagTGGAGTTGCACTCCAACGTGA
pal1-RNAi-R2	catgccaattctagagggcccGATTTGCAACCGTTGTCTTACG
pal2-RNAi-F1	aatttcactgacctggCCACCGACAATCCTCTCATCG
pal2-RNAi-R1	gcacaaccaagcagtaaaCTAGAAAATGAGAATAAGACCTTGCTACC
pal2-RNAi-F2	agTTTACTGCTTGGTTGTGCATTTC
pal2-RNAi-R2	catgccaattctagagggcccCCACCGACAATCCTCTCATCG
hyg F	CGACAGATCCGGTCGGCATCTACTCTATTTCTT	Detection of transformants
hyg R	TCTCGTGCTTTCAGCTTCGATGTAGGAGGG
OE_gpd_pal1F	TGCGTGGTAGAAGAATGG
OE_gpd_pal1R	CGATGAAGAAGGTAGAATGC
OE_gpd_pal2F	CGTTCTCCGAGTCTGTTC
OE_gpd_pal2R	TGATAGCGTCTTGCCATC
pal1-PE-F	TCGCGGATCCGAATTCATGCAATCCTATCCGCA GAC	Construction of prokaryotic expression plasmid
pal1-PE-R	GAGTGCGGCCGCTTAGAATAAATCAACGATATAGGC
pal2-PE-F	CGGATCCGAATTCAGACTATTCTCTAGGGACCACCG
pal2-PE-R	TGCTCGAGTGCGGCCGCCTACGCAACATCGCTACCG