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Effects of sera on a mesenchymal stem cell line. Gene expression profiles confirmed the osteogenic commitment observed in M-CPCs (a); ALP activity (b) and COL1A1 and BMP2 protein expression (c) as well as osteocalcin-positive cells (10x, inset 60x) (d); calcium deposition, evaluated by Alizarin red staining (10x) (e), indicated the increased osteogenic maturation of cells treated with POST RUN sera. The in vitro analyses were performed in three independent experiments, ∗p < 0.05.
