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. 2019 Oct 14;2019:4010764. doi: 10.1155/2019/4010764

Figure 6.

Figure 6

SIRT1 knockdown abolished the protective effects of LEGLTBC. (a) Western blot analysis showed that LEGLTBC silencing or overexpressing positively regulated SIRT1 expression. Knockdown of SIRT1 increased the cleaved caspase 3 expression in HG/PA-treated INS-1 cells (b), which were associated with an obvious increase of apoptotic cells (c) and ROS generation (d). INS-1 cells transfected with vector, LEGLTBC, LEGLTBC+si-NC, and LEGLTBC+si-SIRT1 were treated with HG/PA, followed by the detection of SIRT1 and cleaved caspase 3 expression (e, f, and g) and ROS production (h). Results are presented as means ± SD of n = 3‐5 independent experiments. ∗Conditions significantly different (P < 0.05).