Figure 2.
MSCs at higher passage retain the ability to form spheroids in medium containing KSR. (a) MSCs at P5 and P8 generated spheroids in MiPS at days 1, 3, and 6 on tissue culture dishes. Statistical analysis of the MSC spheroid mean diameter cultured in MiPS; (b) MSCs at P5 and P8 generated spheroids in L-KSR at days 1, 3, and 6. Statistical analysis of the MSC spheroid mean diameter cultured in KSR; sizes were measured from captured images of spheroids (n = 12-20); values are mean ± SD (n = 3). Not significant (NS); P ≥ 0.05 and ∗P < 0.05. Scale bars:100 μm.
