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. 2019 Oct 15;2019:9274585. doi: 10.1155/2019/9274585

Figure 5.

Figure 5

hUC-MSC transplantation decreases GFAP, iba-1, and TLR4 expression in the retinas of OHT-induced rats. Two weeks after hUC-MSC injection. (a–c) TLR4 immunohistochemistry staining from normal eyes, OHT+PBS eyes, and OHT+hUC-MSC eyes; (d–f) amplification of the corresponding groups. The arrow indicates positive TLR4 staining. (g) Quantification of TLR4 staining in retinas (n = 4/group; ∗P < 0.05 and ∗∗P < 0.01 compared with one-way ANOVA with Turkey's post hoc tests). (h) Relative TLR4 gene expression that was normalized to GAPDH in retinas from normal eyes, OHT+PBS eyes, and OHT+hUC-MSC eyes (n = 4/group; ∗∗∗P < 0.001 compared with one-way ANOVA with Turkey's post hoc tests). (i) Western blot analysis for TLR4 protein from normal eyes, OHT+PBS eyes, and OHT+hUC-MSC eyes. (j) Relative TLR4 protein expression that was normalized to GAPDH (n = 4/group; ∗P < 0.05 and ∗∗P < 0.01 compared with one-way ANOVA with Turkey's post hoc tests). (k) Western blot analysis of GFAP and iba-1 protein from normal eyes, OHT+PBS eyes, and OHT+hUC-MSC eyes. (l and m) Relative GFAP and iba-1 protein expression that was normalized to GAPDH (n = 4/group; ∗P < 0.05 and ∗∗P < 0.01 compared with one-way ANOVA with Turkey's post hoc tests). Scale bar: 100 μm.