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. 2019 Oct 15;2019:5198138. doi: 10.1155/2019/5198138

Figure 2.

Figure 2

Cysteine 151 is required for ATT-induced modification to Keap1. (a) COS1 cells were transfected with Keap1-expressing plasmid and treated with varying doses of ATT for 16 hours. Immunoblots show Keap1 and GAPDH levels. (d) COS-1 cells were transfected with vectors expressing either mutant Keap1 (lanes 1 to 12) or wild-type (WT) Keap1 (lanes 13–14) and treated with either 7 μM ATT (+) or DMSO (−) for 16 hours. (b-c) Comparison of the expression levels of modified Keap1 and keap1-69KD at different does of ATT. All values represent mean ± SD of three experiments; ∗P < 0.05; ∗∗P < 0.01.