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. 2019 Oct 13;2019:1681972. doi: 10.1155/2019/1681972

Figure 4.

Figure 4

Effects of F. nucleatum on ROS generation in GFs. (a) Flow cytometry analysis of ROS production with F. nucleatum stimulation (MOIs of 0, 10, 50, 100, 200, and 400) at 2 h, 6 h, 12 h, 24 h, and 36 h. (b) Venn diagram of ROS production-related DEGs from the GO biological process analysis after the five paired comparisons. (c) Statistical results of ROS generation by flow cytometry (n = 3). (d) The relative gene expression level of SOD2 by qRT-PCR. The histogram represents the mean ± SD. Statistical analyses were performed by two-way ANOVA with Turkey's multiple-comparison test (c) and multiple t-test (d). ∗P < 0.05 and ∗∗∗P < 0.001 compared with the control at each time point.