Fig. 2.

Cacna1s expression in the retina. (A) Schematic of genome coverage of RNAseq data (Brooks et al. 2011 and Sandstrom, 2012) at the Cacna1s locus. The y-axis represents expression level as FPKM. (B) Schematic diagram of skeletal muscle and retina splicing pattern for Cacna1s, as indicated by analyses of the RNAseq alignment data. The retina uses two previously undescribed exons, referred to here as 16a and 16b. (C) qRT-PCR analyses of Cacna1s expression in skeletal muscle and retina. Assays were located at the indicated exon junctions. There is no expression of Cacna1s exons 1-16 in retina. Data were normalized to 18S expression. Note the difference in scales used for skeletal muscle and retina. (D) Amplified full-length transcript from skeletal muscle and retina revealed that the retinal isoform of Cacna1s is 2,224 bp smaller than the skeletal muscle isoform.