Figure 4:

The nonpsychoactive cannabinoids CBDV, CBG, CBC, CBN and CBD all expedite the rate of removal of aggregated Aβ. MC65 cells were induced to make Aβ for 24 hrs (I24) or non-induced (NI). At this point, the cells were exposed to 100 nM cannabinoid, with or without tet and a γ secretase inhibitor (SI) (1 μM). Cells were harvested 24 hr later. Six lanes were with tet and SI and 5 lanes with the 5 cannabinoids. For statistics, all lanes were scanned for band density and the six control lanes were compared to the 5 treated lanes. (A) Western blot with the 6E10 antibody. (B) Bar graph of the quantified Western blot data. CAN is the sum of the cannabinoids.