Table 2.
Pharmacology
| MC65 | HT22 | ||||
|---|---|---|---|---|---|
| Compounds | MOA | Activity | EC50 | Activity | EC50 |
| R-1 methanandamide | CB1 agonist | protects | 3 μM | protects | 4 μM |
| 2 AG | CB1/2 agonist | protects | 0.05 μM | potentiates | 0.35 μM |
| Arvanil | CB1 agonist | protects | 0.4 μM | potentiates | 0.1 μM |
| AEA | Endocannabinoid | protects | 0.3 μM | protects | 0.35 μM |
| AM 404 | AEA analogue | protects | 0.4 μM | protects | 10 μM |
| SLV319 | CB1 antagonist | no effect | — | no effect | — |
| AM 281 | CB1 antagonist | potentiates | 0.9 μM | potentiates | 1.5 μM |
| AM 251 | CB1 antagonist | potentiates | 3.5 μM | potentiates | 1 μM |
| CP-55, 940 | CB1 and 2 agonist | protects | 5 μM | protects | 6 μM |
| NADA | CB1 and CB2 agonist | protects | 0.08 μM | protects | 1.1 μM |
| WIN-55, 212 | CB2 agonist | protects | 1.5 μM | no effect | — |
| JWH 133 | CB2 agonist | no effect | >10 μM | no effect | — |
| Q-3-Carboxamide | CB2 agonist | protects | 0.09 μM | no effect | — |
| AM 1241 | CB2 agonist | protects | 0.3 μM | protects | 0.45 μM |
| AM 630 | CB2 antagonist | potentiates | 0.01 μM | potentiates | 0.3 μM |
| UBR 597 | Inhibits AEA degradation | protects | 1.1 μM | protects | 0.5 μM |
| RCS-8 | CB1 agonist | no effect | — | no effect | — |
| MDA-19 | CB2 agonist | no effect | — | no effect | — |
| MCBN | Methyl ether of CBN | no effect | — | no effect | — |
| Hu210 | CB1 agonist | protects | 0.95 μM | protects | 0.6 μM |
| Hu211 | Inactive enantomer of Hu210 | protects | 1.5 μM | protects | 0.72 μM |
MC65 cells were induced to make Aβ or HT22 cells were exposed to a toxic glutamate concentration in the presence of three-fold serial dilutions between 10 nM and 20 μM of the indicated compounds. Cell viability was monitored and the compounds either had no effect, potentiated, or inhibited toxicity. There was no direct toxicity to cells at the concentrations indicated. The EC50S are given for either protection or potentiation. NADA is N-arachidonoyl dopamine, Q-3-carboxamide is 4-quinolone-3 carboxamide, and AEA is arachidonyl ethanolamide, 2 AG is arachidonoylglycerol, and MCBN is methyl CBN. (—) means no value determined.