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Nanoparticle preparation and characterization. (A) Schematic illustration of PLGA-PEG NPs (top) and SPNs (bottom). (B) Particle concentration quantified through nanoparticle tracking analysis (NTA). (C, D) Particle size distribution and zeta-potential quantified by dynamic light scattering (DLS) analysis. (E) Brightfield (left) and confocal fluorescent microscope (right) images of SKBR3 and MDA-MB-231 cell lines with (bottom) and without (top) incubation with SPNs. Scale bar: 100 μm.
