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. Author manuscript; available in PMC: 2021 Jan 10.
Published in final edited form as: Prog Neuropsychopharmacol Biol Psychiatry. 2019 Aug 12;96:109730. doi: 10.1016/j.pnpbp.2019.109730

Figure 3.

Figure 3.

Effects of five days of daily bilateral intra-bed nucleus of the stria terminalis (BNST) microinjections of vehicle (n = 11, open circles) or urocortin 1 (UCN1, n = 11, closed circles) on slc6a4 mRNA expression in subdivisions of (A) the dorsal raphe nucleus (DR), (B) in the entire DR (average of all subdivisions), and (C) in the median raphe nucleus (MnR). Slc6a4 mRNA expression is displayed throughout the entire rostrocaudal extent of each subdivision, with bregma levels (+7 through −6) on the x-axis, and gene expression on the y-axis. Borders between rostral and caudal aspects of each subdivision are indicated with dashed vertical lines. (D-E) Bar graphs displaying the overall expression of slc6a4 mRNA expression in the DRI and rostral MnR (rMnR). Shown is the mean ± or + the standard error of the mean (SEM). Representative photomicrographs show slc6a4 mRNA expression in the (F) rostral DRD core at approximately −7.916 mm from bregma, (G) the rMnR at approximately −7.916 mm bregma, and (H) in the dorsal raphe nucleus, interfascicular part (DRI) at approximately −8.588 mm bregma of vehicle-and Ucn1-primed rats. The white arrow indicates the core of the rostral aspect of the dorsal raphe nucleus, dorsal part (rDRD core). Abbreviations: DRC, dorsal raphe nucleus, caudal part; DRD, dorsal raphe nucleus, dorsal part; DRI, dorsal raphe nucleus, interfascicular part; DRV, dorsal raphe nucleus, ventral part; DRVL/VLPAG, dorsal raphe nucleus, ventrolateral part/ventrolateral periaqueductal gray; rMnR, rostral aspect of the median raphe nucleus; Scale bar: 1 mm. p* < 0.05, p** < 0.01, compared to vehicle controls.