Figure 3.

LINC01225 regulated EMT process through Wnt/β‐catenin signalling pathway. A, RT‐qPCR analysis of the main EMT markers E‐cad, ZO1, N‐cad, vimentin, FN1, MMP1, MMP2, MMP7, MMP9 as well as myc and cyclin D1 in LINC01225‐silencing GC cells. B, RT‐qPCR analysis of the main EMT‐inducing transcription factors Snail, Twist, ZEB1, Slug as well as Wnt‐related genes in LINC01225‐silencing GC cells. LINC01225‐silencing GC cells were co‐transfected with si‐GSK‐3β, or treated with GSK‐3β inhibitor LiCl. Relative expression of EMT markers and Wnt‐related genes were determined by RT‐qPCR (C, D) or Western blot (E). *P < .05 vs si‐NC, #P < .05 vs si‐LINC01225