Figure 6.

ELF5 binds to EHF locus and represses gene expression. (A) mRNA expression analysis of EHF and ELF5 during siRNA‐mediated depletion of ELF5. Error bars are SEM, n = 3. ****P < .0001, ***P < .001, **P < .01, *P < .05, ns = not significant by an unpaired Student's t test. (B) Western blot showing increase in EHF protein levels in response to transfection of T47D cells with siRNA against ELF5. (C) Open chromatin and ELF5 occupancy across 11p13 in T47D cells; ATAC‐seq analysis, upper track and sites of ELF5 occupancy identified by ELF5 ChIP‐seq, lower track. (D) ChIP followed by qPCR analysis confirming enrichment of ELF5 at multiple sites (marked) within the EHF locus. Error bars are SEM, n = 3. ****P < .0001, ***P < .001, **P < .01, *P < .05, ns = not significant by an unpaired Student's t test