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. 2019 Aug 30;23(11):7673–7684. doi: 10.1111/jcmm.14640

Figure 1.

Figure 1

The preparation of native stromal cell‐derived factor‐1 (SDF‐1), AnxA5 and SDF‐1‐Annexin. A, Schematic illustration of SDF‐1‐AnxA5 sequence. Cys316 to Ser is mutation site to avoid mismatching disulfide bones of SDF‐1. B, Agarose gel electrophoresis analysis of SDF‐1, Linker, AnxA5 and SDF‐1‐AnxA5. C, SDS‐PAGE analysis of expression and purification of SDF‐1, AnxA5 and SDF‐1‐AnxA5. D, Native SDF‐1, AnxA5 and SDF‐1‐AnxA5 were identified by Western blot with anti‐SDF and anti‐AnxA5 antibodies