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. 2019 Oct 23;12:8725–8736. doi: 10.2147/OTT.S219900

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© 2019 Shi et al.

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Overexpression of CASC15 arrested cell growth, migration and invasion, and promoted cell apoptosis in ovarian cancer cells in vitro. (A) CASC15 sequences were binded into the pEX-2 vector (pEX-CASC15). An empty pEX-2 vector served as a negative control (pEX). QRT-PCR assay analyzed the expression of CASC15 in OVCAR3 and SKOV3 cells transfected with pEX-CASC15 or pEX. **P < 0.01. (B and C) Overexpression of CASC15 significantly reduced cell proliferation of ovarian cancer cells by CCK-8 assay. **P < 0.01. (D) Soft-agar assay indicated that overexpression of CASC15 significantly abrogated the colony-forming capacity of ovarian cancer cells. **P < 0.01. (E) Overexpression of CASC15 obviously arrested cell cycle at G0/G1 stage by cell cycle assay. (F) Overexpression of CASC15 dramatically facilitated cell apoptosis by caspase-3 ELISA assay kit. (G) Overexpression of CASC15 markedly diminished migration and invasion of ovarian cancer cells by transwell assay. *P<0.05, **P<0.01. Data were shown as mean ± SD, and all experiments were performed in triplicate.