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. 2019 Oct 23;12:8725–8736. doi: 10.2147/OTT.S219900

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© 2019 Shi et al.

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CASC15 directly interacts with miR-221. (A) Bioinformatic analysis was used to predict binding sites between CASC15 and miR-221. (B) The relative expression of miR-221 in tumor tissues was prominently augmented compared to normal tissues. (C) Strong negative correlation between miR-221 and CASC15 expression in ovarian cancer tissues by Pearson correlation analysis. (D) Dual-luciferase reporter assay was performed in the HEK-293 cells transfected with the reporter vector wt-CASC15, not mut-CASC15. (E) Relative expression of miR-221 was measured by qRT-PCR in ovarian cancer cells transfected with pEX-CASC15 or pEX. (F) Relative expression of CASC15 in ovarian cancer cells transfected with anti-miR-221 was detected by qRT-PCR. (G) Detection of miR-221 using qRT-PCR in the sample pulled down by biotinylated CASC15 probe. *P<0.05, **P<0.01. Data were shown as mean ± SD, and all experiments were performed in triplicate.