Figure 2.

miR‐206 overcame IL‐6‐induced gefitinib resistance in PC‐9 and HCC827 cells. A, cells were treated with gefitinib for 24 h to measure viability by CCK‐8 assay. B, cells were treated with 0.1 μmol/L gefitinib and/or 20 nmol/L miR‐206 mimics for 6 h to measure apoptosis by flow cytometry. C, cells were treated with 0.1 μmol/L gefitinib and/or 20 nmol/L miR‐206 mimics for 7 d to measure gefitinib‐resistant colony formation. PC‐9 and HCC827 cells were cultured for 72 h with 10 ng/mL rhIL‐6 prior to gefitinib or mimics treatment. The mean ± SD values are shown. **P < .01