Figure 3.

Forced miR‐206 expression blocked IL‐6/Jak1/Stat3 cascade. A, IL‐6‐activated Jak1‐Stat3 signalling was determined by Western blot. B‐C, miR‐206 mimics abolished IL‐6‐induced p‐Stat3 and p‐Jak1. PC‐9 cells were cultured for 72 h with 10 ng/mL rhIL‐6 prior to mimics treatment. D, colivelin significantly abrogated miR‐206 increased gefitinib sensitivity in PC‐9 and HCC827 cell; PC‐9 cells were transfected with 20 nmol/L miR‐206 mimics or 20 nmol/L miR‐206 mimics plus 50 nmol/L colivelin along with 0.1 μmol/L gefitinib treatment for 6 h to measure apoptosis by flow cytometry, the control group was only treated with 0.1 μmol/L gefitinib, and the cell viability was normalized to the control group. The min to max values and mean ± SD values are shown. *P < .05, **P < .01, ***P < .001