Figure 3.

Sodium hydrosulphide (NaHS) improved fatty acid dysregulation in tumour necrosis factor (TNF)‐α‐treated HL‐1 cells. Western blot analysis of 5′ adenosine monophosphate‐activated protein kinase (AMPK) 2α, phosphorylated AMPK2α (pAMPK2α), phosphorylated acetyl coenzyme A carboxylase (pACC), peroxisome proliferator‐activated receptor‐γ coactivator‐1α (PGC‐1α), carnitine palmitoyltransferase 1 (CPT‐1) and diacylglycerol acyltransferase 1 (DGAT1) expression from cells treated with TNF‐α (25 ng/mL) or NaHS (0.1 mmol/L) combined with TNF‐α for 24 h. Densitometry was normalized to glyceraldehyde 3‐phosphate dehydrogenase (GAPDH) as an internal control. Data are shown as mean ± SEM from four independent experiments