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. 2019 Sep 9;23(11):7830–7843. doi: 10.1111/jcmm.14662

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© 2019 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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The impact of MCU inhibition and activation on ATP production, mitochondrial fission, fusion and mitophagy. A, ATP production. B and C, Protein expression of mitochondrial fission, fusion and mitophagy by Western blot assay and quantitative analysis. D‐F, Mitochondrial morphology by MitoTracker Green staining and confocal microscopy of primary cardiomyocytes and quantitative analyses after 12h/3h H/R, with or without Ru360/spermine treatment, and quantitative analysis. The scale of the mitochondrial images is 10µm. Data are means ± SD from at least three different experiments. *P < .05, **P < .01