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. 2019 Sep 9;23(11):7773–7784. doi: 10.1111/jcmm.14654

Figure 3.

Figure 3

Testis developmental related gene 1 (TDRG1) and autophagy regulate cell viability and apoptosis in response to cis‐dichlorodiammine platinum (CDDP) treatment in TCam‐2 cells. A, MTT assays were used to confirm the effects of different concentrations of CDDP on the viability of TCam‐2 cells. B, MTT assays were performed to confirm the effects of different expression levels of TDRG1 on the viability of TCam‐2 cells following CDDP(IC25) treatment. C, MTT assays were used to confirm the effects of autophagy inhibition with 3‐MA on the viability of TCam‐2 cells following CDDP(IC25) treatment. D, Left: Cells were treated with different concentrations of CDDP or subjected to up‐ or down‐regulation of TDRG1 or treated with the autophagy inhibitor 3‐MA for 72 h, followed by staining with Annexin V‐fluorescein isothiocyanate and propidium iodide and analysed by flow cytometry. Right: Quantitative summary of the early apoptosis rate in the different treatment groups. Compared as indicated. (Data from three independent experiments, error bars represent SD, *P < .05, **P < .01, ***P < .001)