Figure 4.

Testis developmental related gene 1 (TDRG1) regulates chemosensitivity of TCam‐2 cells to cis‐dichlorodiammine platinum (CDDP) through autophagy. A, Immunofluorescence images of LC3‐II (red) in TCam‐2 cells treated with different concentrations of CDDP or subjected to up‐ or down‐regulation of TDRG1 or treated with the autophagy inhibitor 3‐MA for 72 h. B, Protein expression of TDRG1, p110β, LC3‐II and active caspase 3 in TCam‐2 cells with or without Baf A1 treatment was measured by Western blotting at 72 h after treatment with different concentrations of CDDP or with different expression levels of TDRG1 or autophagy inhibition with 3‐MA. GAPDH served as an internal control. (Data from three independent experiments, error bars represent SD, *P < .05, **P < .01, ***P < .001)