Figure 5.

Testis developmental related gene 1 regulates seminoma growth through autophagy in response to cis‐dichlorodiammine platinum treatment in vivo. A, Images of representative tumours in nude mice from different treatment groups and growth curves of transplanted tumours in nude mice (n = 6). B, Protein lysates were collected from mouse xenograft tumours from different treatment groups, and the levels of TDRG1, LC3‐II and active caspase‐3 were measured by Western blotting. GAPDH served as a loading control. C, Representative images of immunohistochemistry analysis of Ki‐67, magnification 200×. The expression index of Ki‐67 in the different groups was analysed (error bars represent SD, *P < .05, **P < .01, ***P < .001)