Figure 3.

ERβ deficiency results in more adipocyte accumulation and activation of PPARγ signalling pathway. (A,B) Oil Red O staining represented adipocytes in tendon scar region. C, Adipocytes labelled by Oil Red O staining were significantly increased in ERβ^−/− mice compared with WT controls. (D,E) Immunofluorescence of CD34‐positive adipocytes in tendon scar region. F, CD34‐positive adipocytes were significantly increased in ERβ^−/− mice compared with WT control. G, ERβ^−/− mice tendon scars displayed significantly higher expression levels of CD36, PPARγ, FABP4, adiponectin and Lpl, but no difference compared with WT controls. H, Representative immunoblots of PPARγ signalling pathway including CD36, PPARγ and FABP4 were significantly activated in ERβ^−/− mice tendon scars compared with WT control. Data are represented as mean ± SEM (n = 5), **P < .01, ***P < .001. Scale Bars: 100 μm