Figure 5.
Activation of ERβ inhibits adipogenic differentiation of TDSCs by a negative crosstalk with PPARγ signalling. (A‐C,G) Oil Red O staining and statistic analysis revealed inhibition of adipogenic differentiation of TDSCs by 1 × 10−7 mol/L LY3201, which could be partly re‐activated by the treatment of ROSI. (D‐F,H) Immunofluorescence of PPARγ and statistic analysis showed a consistent change with Oil Red O staining result. J qRT‐PCR revealed the change of mRNA levels related to PPARγ signalling including CD36, PPARγ, FABP4 and Lpl with the treatment of LY3201 and ROSI. (I,K) Representative immunoblots and statistic analysis of PPARγ signalling pathway including CD36, PPARγ and FABP4 with the treatment of LY3201 and ROSI. Data are represented as mean ± SEM (n = 5), **P < .01, ***P < .001. Scale Bars: 100 μm