Figure 4.

Co‐culturing with miR‐20b‐3p‐enriched exosomes alleviates oxalate‐induced injury in NRK‐52E cells by inhibiting inflammatory responses via TLR4. A and B, TLR4 levels in NRK‐52E cells after TLR4 overexpression for 48 h determined by RT‐PCR and Western blotting. C, Cell viability assessed by MTT assay. D, Levels of miR‐20b‐3p and ATG7 determined by RT‐PCR. E and F, Crystal cell adhesion assay determination and quantitation. G, Cellular protein concentrations for IL‐1β, IL‐6 and TNF‐α determined by ELISA. H, Inflammation‐related proteins TLR4, p65 and IkB determined by Western blotting and quantified. Data are presented as the mean ± SD (n = 3). **P < .01 and ***P < .001 vs control group; #P < .05, ##P < .01 and ###P < .001 vs OX group; $P < .05, $$P < .01 and $$$P < .001 vs OX + Exo‐miR‐20b‐3p group