Figure 4.

Effects of overexpression of miR‐20a‐5p on surfactant synthesis. AT‐II cells were seeded in plastic dishes and transduced with adenoviruses carrying a universal sequence (NC), a rat miR‐20a (miR‐20a), then cultured for 2 d. Total RNA or protein was extracted. Real‐time PCR was done to determine the mRNA abundance of SP‐A, SP‐B, SP‐C and SP‐D. Western blot was used to detect the abundance of SPs. A, miR‐20a‐5p increased the expression of SP‐A mRNA (**P < .01). B, miR‐20a‐5p increased the expression of SP‐B mRNA (****P < .0001). C, miR‐20a‐5p increased the expression of SP‐C mRNA (*P < .05). D, miR‐20a‐5p increased the expression of SP‐D mRNA (**P < .01). E, Western blots confirm increased SP levels with overexpression of miRNA‐20a‐5p in AT‐II. Left lanes: negative control (NC); right lanes: overexpressed miRNA‐20a‐5p