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. 2019 Oct 15;11(10):842–856. doi: 10.4251/wjgo.v11.i10.842

Table 2.

Primer/mimic sequences

Name Sequence (5’–3’)
miR-320a reverse transcription primer
miR-320a GTCTGTATGGTTGTTCTCGACTCCTTCACATCCCTATCCAACCATACAGACTCGCCCTC
U6 snRNA GGAACGCTTCACGAATTTG
Real-time PCR primer sequence
miR-320a FO:AAGGGATCGCGGGCG
RE:TGCGTGTCGTGGAGTC
U6 snRNA FO:ATTGGAACGATACAGAGAAGATT
RE:GGAACGCTTCACGAATTTG
MSP primer sequence
Methylated FO:ACGTCGTAATGTGAGGATTC
RE:CGCAAACAAAACTCGATATAA
Nonmethylated FO:ATTATGTTGTAATGTGAGGATTT
RE:CACAAACAAAACTCAATATAACCC
miR-320a mimics sequence
miR-320a Sense: AAAAGCUGGGUUGAGAGGGCGA
Antisense: GCCCUCUCAACCCAGCUUUUUU
Negative control Sense: UUCUUCGAACGUGUCACGUTT
Antisense: ACGUGACACGUUCGGAGAATT
PBX3 primer sequence FO:GCCAAATTGACCCAGATCAGAC
RE:GAAATGGGACGTGTTCTACTCTGTT

PCR: Polymerase chain reaction.