Table 2.
Name | Sequence (5’–3’) |
miR-320a reverse transcription primer | |
miR-320a | GTCTGTATGGTTGTTCTCGACTCCTTCACATCCCTATCCAACCATACAGACTCGCCCTC |
U6 snRNA | GGAACGCTTCACGAATTTG |
Real-time PCR primer sequence | |
miR-320a | FO:AAGGGATCGCGGGCG |
RE:TGCGTGTCGTGGAGTC | |
U6 snRNA | FO:ATTGGAACGATACAGAGAAGATT |
RE:GGAACGCTTCACGAATTTG | |
MSP primer sequence | |
Methylated | FO:ACGTCGTAATGTGAGGATTC |
RE:CGCAAACAAAACTCGATATAA | |
Nonmethylated | FO:ATTATGTTGTAATGTGAGGATTT |
RE:CACAAACAAAACTCAATATAACCC | |
miR-320a mimics sequence | |
miR-320a | Sense: AAAAGCUGGGUUGAGAGGGCGA |
Antisense: GCCCUCUCAACCCAGCUUUUUU | |
Negative control | Sense: UUCUUCGAACGUGUCACGUTT |
Antisense: ACGUGACACGUUCGGAGAATT | |
PBX3 primer sequence | FO:GCCAAATTGACCCAGATCAGAC |
RE:GAAATGGGACGTGTTCTACTCTGTT |
PCR: Polymerase chain reaction.