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. 2019 Nov;371(2):278–289. doi: 10.1124/jpet.119.257592

Fig. 4.

Fig. 4.

Intravenous push infusion of DOX disrupts lymph flow in rat mesenteric lymph vessels in vivo. (A) HPLC chromatogram reveals a retention time of 4.36 minutes for DOX. Daunorubicin (DAUN) was used as an internal standard and eluted at 9.21 minutes. (B) DOX (10 mg/kg) was administered via rapid tail-vein infusion and blood collected and analyzed by HPLC as described in Materials and Methods. An initial rapid elimination phase of approx. 45 minutes was followed by a plateau phase corresponding to tissue release. Gray shading denotes clinically achievable plasma concentrations of DOX at ≤4 hours after an intravenous push infusion. Data reported as mean ± S.E.M. (n = 5). (C) Compared with saline, systemic administration of DOX significantly and persistently decreased positive volumetric flow in rat mesenteric lymph vessels [F(1,14) = 35.53]. Data reported as mean ± S.E.M. and analyzed using two-way ANOVA with Holm-Sidak post-test (n = 8; ****P < 0.0001).