Skip to main content
. 2019 Sep 19;9(23):6745–6763. doi: 10.7150/thno.34674

Figure 1.

Figure 1

Effect of CA on Huh7 and H446 cells. (A) Chemical structure of CA. (B) Increased doubling time of Huh7 and H446 cells after treatment with CA (25 or 50 µM) on day-4. (C) The representative image of invading cells after exposure to CA (25 or 50 µM; 24 h. Scale bars indicate 10 μm). (D) The representative image of migrating cells after exposure to CA (25 or 50 µM; 24 h. Scale bars indicate 10 μm). (E) CA-induced morphological change in cellular nuclei (CA at 25 or 50 µM; 48 h). Red arrow indicates cell karyopyknosis (stained with DAPI; 400 ×; Bar, 10 μm). (F) Cell cycle analysis of Huh7 and H446 cells treated with CA (25 or 50 μM for 24 h). Compared with the solvent control, the number of cells in the G0/G1 phase increased from 55.21% to 63.75% (CA 25 μM) or 70.95% (CA 50 μM) in Huh7 cells, and from 65.49% to 78.38% (CA 25 μM) or 82.79% (CA 50 μM) in H446 cells. S-phase cells decreased from 26.26% to 19.22% (CA 25 μM) or 10.68% (CA 50 μM) in Huh7 cells and from 30.31% to 17.56% (CA 25 μM) or 13.72% (CA 50 μM) in H446 cells. All data are expressed as mean ± SEM. The experiments were done 3 times. * p < 0.05, *** p < 0.001.