Isolation and characterization of fibrogenic fibroblasts by IR-780. (A) the subcellular localization of IR-780 in skin fibroblasts. Scale bar, 25 µm. (B) the differentially accumulation of R-780 in the human skin fibroblasts. Scale bar, 75 µm. (C) Schematic of the experimental strategy for isolation and characterization of IR-780H fibroblasts. (D) the NIR images of newly isolated IR-780H and control fibroblasts. Scale bar, 75 µm. (E) the morphologic images of IR-780H and control fibroblasts. Scale bar, 100 µm.(F) Immunostaining of IR-780H and control fibroblasts for COL1A1. Scale bar, 100 µm.(G) western blots, (H) real-time RT-PCR and (I) gene expression profile analyzing the fibrogenic genes in IR-780H and control fibroblasts.