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. 2019 Sep 19;9(23):6797–6808. doi: 10.7150/thno.36375

Figure 5.

Figure 5

In vitro photoinduced cytotoxicity of IR-780H fibroblast population. (A) Calcein AM/ PI staining of IR-780H and control fibroblasts exposed to 0.4 µM IR-780 with NIR laser of 1.25 W cm-2. Scale bar, 100 µm. (B) Apoptosis detection of human IR-780H and control fibroblasts exposed to 0.4 µM IR-780 with NIR laser irradiation (808 nm, 1.25 W cm-2) by Flow Cytometry. (C) Calcein AM/ PI staining and (D) Cell viability tests of IR-780 treated primary fibroblasts with or without ice, NAC and NIR laser. **, P<0.01. (E)Singlet oxygen generation of IR-780 exposed to NIR laser, (F) the ROS generation of fibroblasts treated with 0.4 µM IR-780 and NIR laser. **, P<0.01. (G) Real time thermal images of IR-780 solution irradiated with NIR laser.