IONzyme-loaded facemask protects against influenza. (A) A picture of anti-IAVs mask with eight-layer absorbent filter cotton. Different concentrations of IONzymes were added on the third layer of selected area (5 cm2), respectively. After drying, IAVs (H1N1, H5N1, and H7N9 subtype) was sprayed onto the eighth layer (the outermost layer). After standing for 0.5 or 1 h, the population of the living virus on the control area or IONzyme-added area were harvested, and then HA and TCID50 titers of H1N1 (B), H5N1 (C), and H7N9 (D) IAVs were measured. The data are presented as the mean ± s.d. of three independent experiments. Statistical significance is assessed by unpaired Student's two-sided t-test to the control group. **P<0.01.