Figure 7.

Chick ex ovo culture using chicken CAM assay. (A) Schematic time line of the ex ovo CAM procedure. (B) Representative images taken on EDD 13 showed CAM vascularization responsive to different treatments including untreated negative control, GF-free hydrogel, GF-loaded hydrogel, and free GF applied on filter paper as a positive control. The semi-automatic processed images were also provided for quantitative analysis. Scale bars indicate 2 mm. (C) Hydrogels loaded with 200 ng of VEGF and HGF, respectively, were grafted on the outer region of shell-less chicken embryo CAM. (D) Quantification of total vessel length surrounding the gel (the values were normalized with the analyzed area). * indicates p < 0.05, ** indicates p < 0.01, and NS denotes no significant difference. Error bars show ± S.D. for total n = 6.