Figure 4.

Graphical representation of clonal evolution from pre- to post-CRT cancer. (A) and (B) show two clonal evolution patterns, branched and linear, as observed in two patients, ID27 and ID9, respectively. Top graph shows cellular prevalence of mutations after clustering using PyClone. Bottom graph exhibits the visualization of tumor evolution using Timescape. Each color represents a clone. The horizontal axis denotes 3 different time point of tumor, from the time of tumorigenesis (T0) to diagnosis (pre-CRT) and after surgery (post-CRT). In patient ID9, only 2.5% of cancer cells survived during CRT. See also Supplementary Figure S5.