Table 4.
Summary Statistics for Spore Recovery from Sandy Soil in EPA and USGS Labs
| Percent of Positive Control (spores/g) Targeted | Spores/g Actually Achieved | Day | EPA Lab | USGS Lab | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Raw Data Mean CFU/plate Rangea | Percent Recovery (%R) Range | Geometric Mean | Raw Data Mean CFU/plate Rangea | %R Range | Geometric Mean | |||||||
| Lowest Observed Mean CFU/plate (95% CI) | Highest Observed Mean CFU/Plate (95% CI) | Positive Control Mean CFU/plate (95% CI) | Lowest Observed Mean CFU/plate (95% CI) | Highest Observed Mean CFU/plate (95% CI) | Positive Control Mean CFU/Plate (95% CI) | |||||||
| 5% (17.5) | 17.6 | 1 | 3.4-7.4 | 53.7-116.8% | 3.3 (3.2-5.8) | 7.1 (5.7-11.5) | 83.1 (78.0-90.6) | 3.6-6.0 | 56.8-94.7% | 3.6 (4.0-5.3) | 5.5 (3.9-10.9) | 99.5 (94.2-107.1) |
| 2 | 4.9-7.8 | 77.3-123.1% | 4.8 (3.9-8.5) | 7.4 (6.5-10.9) | 106.4 (99.4-116.2) | 3.3-6.1 | 52.1-96.3% | 2.6 (2.1-6.2) | 5.7 (4.9-9.1) | 102.3 (99.0-107.7) | ||
| 5% (17.5) | 19.7 | 1 | 4.4-5.6 | 62.0-79.0% | 4.0 (3.0-8.4) | 5.2 (3.9-9.9) | 88.4 (87.3-91.5) | 4.6-6.4 | 64.9-90.2% | 4.1 (2.4-10.9) | 6.3 (5.9-9.1) | 95.7 (91.0-102.7) |
| 2 | 3.9-6.8 | 55.0-95.9% | 3.6 (3.4-6.2) | 6.3 (5.4-9.9) | 113.8 (105.1-125.3) | 5.1-6.7 | 71.9-94.5% | 4.8 (4.4-7.7) | 6.2 (5.1-10.1) | 118.2 (112.2-126.6) | ||
| 10% (35) | 29.6 | 1 | 6.2-9.8 | 58.2-92.0% | 5.4 (3.3-12.4) | 9.6 (8.4-13.4) | 88.4 (73.7-108.4) | 6.2-13.2 | 58.2-123.9% | 5.9 (4.8-9.9) | 13.1 (12.2-16.3) | 66.3 (63.4-71.5) |
| 2 | 8.6-10.7 | 80.7-100.4% | 8.6 (8.8-10.4) | 10.4 (9.1-14.1) | 96.2 (88.5-106.8) | 8.7-13.4 | 81.6-125.8% | 8.6 (8.5-10.9) | 12.9 (11.2-17.4) | 68.1 (65.7-72.7) | ||
| 10% (35) | 35.6 | 1 | 10.8-13.6 | 84.3-106.1% | 10.2 (7.4-17.0) | 13.2 (10.3-19.5) | 107.6 (97.6-120.9) | 7.6-15.4 | 59.3-120.2% | 7.5 (7.3-10.0) | 15.1 (12.7-20.4) | 112.4 (105.0-122.4) |
| 2 | 10.8-13.5 | 84.3-105.3% | 10.3 (8.7-14.6) | 13.2 (12.0-16.9) | 112.5 (106.7-120.7) | 9.1-10.9 | 71.0-85.0% | 8.7 (7.6-12.4) | 10.6 (9.5-14.1) | 108.0 (99.2-119.7) | ||
| 25% (87.5) | 89.3 | 1 | 20.40-26.8 | 63.5-83.4% | 20.3 (18.2-24.8) | 26.2 (20.7-35.7) | 66.7 (56.1-81.7) | 27.4-33.2 | 85.2-103.3% | 27.1 (23.0-34.3) | 32.9 (28.6-40.2) | 112.3 (98.8-130.0) |
| 2 | 27.6-31.7 | 85.9-98.6% | 26.9 (23.2-33.6) | 31.2 (27.5-37.7) | 93.7 (88.4-101.4) | 26.2-31.2 | 81.5-97.1% | 25.9 (23.5-30.7) | 30.8 (27.7-36.5) | 98.7 (89.7-111.0) | ||
| 50% (175) | 166.7 | 1 | 38.4-47.6 | 61.0-79.3% | 38.3 (36.3-42.6) | 47.2 (40.4-57.4) | 90.0 (78.7-105.3) | 37.8-51.4 | 63.0-85.6% | 37.6 (33.4-44.5) | 51.3 (48.5-56.4) | 105.8 (94.9-120.1) |
| 2 | 45.7-48.9 | 76.2-81.5% | 45.5 (42.9-50.4) | 48.5 (44.2-55.4) | 97.6 (95.4-102.0) | 45.2-52.0 | 75.3-86.6% | 44.9 (41.4-50.8 | 51.6 (47.3-58.5) | 96.9 (93.4-102.7) | ||
a
All plates were inoculated with 200 uL of soil suspension.