Figure 3.

Treatment of Macaques with Antiretroviral Drugs Prior to Collection of PBMCs Leads to a Reduction of Transduction Efficiency with Gammaretroviral Vectors

PBMCs from six animals using cells collected prior to treatment with anti-retroviral drugs (Pre-ART) or during antiretroviral treatment (ART) were used in the 9-day transduction and expansion protocol. On day 9, cells were evaluated for expression of MBL, representing the CAR, and CXCR5 by flow cytometry. A Wilcoxon matched pairs signed rank test was used to determine significance. Colors denote cells from six individual animals. The bar represents the median value.