Figure 4.

The Majority of CD4-MBL CAR/CXCR5 Transduced Cells are T central memory (TCM)

(A) Representative flow cytometry plots of the starting cell population, day 9 mock, day 9 total transduced, and CAR/CXCR5 transduced subset of rhesus PBMCs. For panels 1–3, gates were set on live, CD3+, and CD8+ cells. For the transduced subset, gates were set on live, CD3+, MBL+, and CD8+ cells. Central memory was defined as CD28+CD95+. Effector memory was defined as CD28−, CD95+. (B) The percentages of central memory cells on day 9 in mock and total transduced cells and the CAR/CXCR5 subset of transduced cells from six individual animals. Mean and 95% confidence intervals are shown with bars. (C) Representative flow plot of CCR7 expression in cells identified as CD28+/CD95+. (D) CCR7 expression levels in the central memory subset of mock, total transduced cells and the CAR/CXCR5 subset of transduced cells from six individual animals. Mean and 95% confidence intervals are shown with bars.