Figure 6.

Transduced Cells Demonstrated Specific Migration to the CXCR5 Ligand, CXCL13, In Vitro

Transduced Rhesus macaque PBMCs (one million cells in 100 μL X-Vivo-15 containing 0.1% BSA) were placed in the upper chamber of a transwell plate with a 5.0 μm membrane. The lower chamber contained X-Vivo/1% BSA only or X-Vivo/1% BSA and the chemokine CXCL13 (2.5 μg/mL). After incubating for 4 h at 37°C, cells were collected and counted on a CytoFLEX flow cytometer (Beckman). All samples were normalized with the addition of AccuCheck counting beads. Specific migration was determined by subtracting the number of cells that migrated to X-Vivo/BSA alone from the number that migrated to the chemokine and then dividing by the number of input cells. Colors denote cells from seven individual animals. A Wilcoxon matched pairs signed rank test was used to determine significance. The bar represents the median value.