Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Sep 14;6(5):1027–1040. doi: 10.1002/ehf2.12509

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2019 The Authors. ESC Heart Failure published by John Wiley & Sons Ltd on behalf of the European Society of Cardiology.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

PMC Copyright notice

Effect of either GGPP synthase or Rho kinase inhibition on hVF differentiation and cellular respiration. (A) Representative immunoblot showing the in vitro effect of either GGPP synthase inhibitor digerenyl bisphonate (DGBP, 30 μmol/L) or ROCK inhibitor Y27632 (10 μmol/L) for 72 h on already‐differentiated myofibroblasts (Diff), and corresponding bar graph shows significant de‐differentiation by DGBP without any effect by Y27632. (B) Graphical representation of pooled data of cellular respiration (OCR) from seahorse assays of differentiated hVFs (TGF) and the GGPP synthase‐inhibited (DGBP) groups. Both basal and ATP‐linked OCRs were significantly inhibited by DGBP. (C) Graphical representation of pooled data of OCR showing no significant effect by GGPP or Y27632 on both basal and ATP‐linked respiration. Data are mean ± SD. ^* P < 0.05; one‐way ANOVA.