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. 2019 Sep 14;6(5):1027–1040. doi: 10.1002/ehf2.12509

Figure 7.

Figure 7

Reduced mitochondrial respiration in hVFs from HF patients under statin therapy. (A) Graphical representation of pooled OCR data from Seahorse assay of hVFs isolated from failing heart patients. (B) Bar graphs depicting the mean OCR for each group at basal, following oligomycin, FCCP, and antimycin A. The basal, ATP‐linked, maximal, and spare capacity OCRs were significantly reduced in hVFs from HF + statin vs. HF group, without significant effect on the Olig‐insensitive proton leak‐linked‐related OCR or non‐mitochondrial OCR. (C) Graphical representation of pooled data of extracellular acidification rate (ECAR) from Seahorse assays of hVFs isolated from failing heart patients. (D) Bar graph shows ECAR data (mean ± SD) with no significant difference in ECAR between the two groups, following addition of either oligomycin, FCCP, or antimycin A; n = 6; analysed by unpaired t‐test. Data are mean ± SD. (E) Representative immunoblot with total OXPHOS human antibody coScktail and corresponding bar graph (F) showed significantly reduced Complex V subunit following statin therapy; n = 4, analysed by one‐way ANOVA.