Figure 5.

Two regions in T. brucei vtRNA are accessible for base-pairing interactions. A, diagram depicting the antisense oligodeoxyribonucleotides used in the RNase H cleavage assay. Shown is the antisense probe used for Northern blotting. B, Northern blot analysis detecting vtRNA in the samples treated with RNase H and the indicated oligos. Controls without oligo (+RNase H) and without both oligo and RNase H (−RNase H) are loaded at the beginning. Marker is a ³²P-labeled pBR322 DNA MspI digest.