Figure 8.

Effect of reduction of vtRNA levels on the newly synthesized SL RNA after longer RNA synthesis time in permeabilized cells. A, analysis of newly-synthesized, ³²P-labeled RNA in permeabilized BF cells treated with the MUT-A or VT-A antisense oligos after a 30-min incubation with ribonucleoside triphosphate mixture containing [α-³²P]UTP. SLex, RNA enriched on antisense SL oligonucleotide beads; poly(A), RNA enriched on (dT)₃₉ beads. The positions of pre-mRNA/mRNA, SL RNA, the 3′-end–shortened SL 130 RNA, and the SL RNA fragments are indicated. B, Northern blot analysis of the indicated RNAs in permeabilized cells treated with the MUT-A or VT-A oligos, after incubation for 30 min with only nonradioactive ribonucleoside triphosphates. C, newly synthesized, ³²P-labeled RNA in permeabilized cells treated with the MUT-A or VT-A antisense oligos after a 5-min incubation with ribonucleoside triphosphate mixture containing [α-³²P]UTP. Input, total RNA in the cells; SLin, RNA enriched on beads coupled to antisense oligonucleotide against the SL RNA intron.