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25-Hydroxycholesterol stimulates both ACAT1 and ACAT2 protein expression in vascular smooth muscle cells. A and D, Western blotting analysis of primary VSMC ACAT2 (A) and ACAT1 (D) expression in response to (0.5, 1, and 5 μm) 25HC treatment (48 h). B and E, quantitation of protein expression of ACAT2 (B) and ACAT1 (E). C and F, expression levels of ACAT2 (C) and ACAT1 (F) mRNA in 25HC-treated VSMCs detected by QPCR. The data are reported as means ± S.E. *, p ≤ 0.05.
