Figure 5.

E2 induced endothelial H₂S release through cGMP. A, HUVECs were treated with E2 at different time points; the level of cGMP was measured by ELISA (mean ± S.D.; n = 3 independent experiments; *, p < 0.05; **, p < 0.01; ***, p < 0.001 versus CON; one-way ANOVA followed by the post hoc LSD test). B, HUVECs were treated with different concentrations of cGMP-PET for 15 min; the cell content of p-VSAP was detected by Western blotting. The cell content of CSE phosphorylation level and total CSE were detected by immunoprecipitation and Western blotting. C, HUVECs were treated with different concentrations of cGMP-PET for 15 min, the medium H₂S concentrations were measured (mean ± S.D.; n = 3 independent experiments; ***, p < 0.001 versus CON, one-way ANOVA followed by the post hoc LSD test).