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PydD activity assays. A, detection of l-glutamate as a reaction product of PydD-catalyzed β-alanine:2-oxoglutarate transamination. Assays monitor NADH formation accompanying l-glutamate oxidation by GLDH. B, coupled activity assays for PydD, detecting malonic semialdehyde generated by PydD-catalyzed β-alanine:2-oxoglutarate transamination. Assays monitor NADPH consumption accompanying malonic semialdehyde reduction by PydE. Error bars, S.E.
