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Michaelis–Menten kinetic parameters of PydE (error values reported are the S.E. for the fits)
| Enzyme | Substrate | kcat | Km | kcat/Km |
|---|---|---|---|---|
| s−1 | mm | m−1 s−1 | ||
| PydE | NADP+; 3-HP | 4.23 ± 0.07 | 7.4 ± 0.4 (3-HP) | 570 ± 30 |
| PydE | NADP+; isethionate | 3.07 ± 0.09 | 55 ± 3 (isethionate) | 56 ± 4 |
| PydE | NAD+; 3-HP | 3.4 ± 0.2 | 4.0 ± 0.7 (NAD+) | 680 ± 160 |
| PydE | NADP+; 3-HP | 2.9 ± 0.1 | 3.4 ± 0.5 (NADP+) | 840 ± 130 |
