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Immunogold labeling of TcpB at pilus ends. Purified TCP were adhered to carbon-coated copper grids, treated with chicken anti-TcpB antibody raised against ΔN-TcpB (Table 2) and gold-labeled secondary antibody, then stained with uranyl acetate, and imaged by transmission EM. Single (A) and multiple (B) gold labels are seen at ends of TCP. Magnification of insets is indicated.
